Treatment of primary root carious lesions using ozone in VIVO


(Division of Restorative Dentistry, Queen"s University Belfast, Northern Ireland, UK)

The aim of this study was to assess the antimicrobial effect of ozone from a novel ozonegenerating device (HealOzone, USA) on primary root carious lesions (PRCLs). 70 PRCLs in 26 patients were randomly selected for the application of ozone either for 10 or 20 s. Each PRCL was classified in terms of colour, texture, cavitation, size, hardness and severity. Overlying plaque was then removed using a hand held standard fine nylon fibre sterile toothbrush with water as a lubricant. Each tooth was dried using dry sterile cotton wool rolls and a dental 3 in 1-air syringe. Half of each PRCL was removed using a sterile excavator. Subsequently, the remaining lesions were exposed to ozone gas for a period of either 10 s (n: 35) or 20 s (n: 35) of ozone at room temperature (23°C) and a further biopsy was taken. Each sample was immediately put into a preweighed sterile vial and weighed. 1 ml of fastidious anaerobe broth (FAB) was added and vortexed for 30 s. Dilutions were performed by transferring 1 ml of the resulting suspensions into 9 ml of FAB and this process was repeated in 10 fold dilution to 104.

After decimal dilution with FAB,  100 μl aliquots (for both test and control groups) were spread on fastidious anaerobe agar (FAA) supplemented with 5% (v/v) horse blood and placed in an anaerobic chamber at 37°C for four days. There were no adverse events observed. Using the Paired Student t tests, a significant (p < 0.00001) reduction (mean ± SE) was observed in the ozone-treated samples with either a 10 seconds (log10 4.35 ± 0.49) or 20 seconds (log10 0.46 ± 0.25) ozone application compared with the control samples (log10 7.00 ± 0.24) and (log10 6.00 ± 0.20) respectively. Using Duncan"s multiple range tests, reduction of total microorganisms after treating test samples with ozone for a period of either 10 or 20 s showed significant differences between severity indices 1 and 2 (p < 0.05). In conclusion, this novel treatment regime using ozone may therefore be considered to be potentially an effective alternative to conventional “drilling and filling” for the management of PRCLs.



Aylin Baysan

St. Bart"s and the Royal London

School of Medicine and Dentistry

Turner St. E1 2AD, London, UK

Tel: +44 20 7377 7000 ext. 2186

Fax: +44 20 7377 7061



ผู้ตั้งกระทู้ Songkhram :: วันที่ลงประกาศ 2011-05-15 06:47:05

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